Hotspot

psbE-petL

LSC · 63409-64193 · 785 bp

Back to Plantago

Pi 0.0487

MarkerSeek score 80.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 209
pi: 0.0487
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AAGCGCTCTTAGTTCAGCTC	45.0	50.0	58.0	58.0	1450-1744 bp	1.0	91.9
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AAGCGCTCTTAGTTCAGCTC	45.0	50.0	58.0	58.0	1449-1743 bp	1.0	91.9
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1427-1721 bp	1.0	91.7
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1426-1720 bp	1.0	91.7
psbE-petL_p5	TAAGCCGTGAATAGCCAACC	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	1558-1852 bp	0.957447	90.0