Hotspot

petA-psbJ

LSC · 61579-62626 · 1048 bp

Back to Plantago

Pi 0.0705

MarkerSeek score 75.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 327
pi: 0.0705
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGGATCTGTTGTTTTGGCA	CCGGATTAGGTTCATCTCTGT	45.0	47.6	57.9	56.9	942-1157 bp	1.0	74.5
petA-psbJ_p2	TGGGATCTGTTGTTTTGGCA	TCCGGATTAGGTTCATCTCTGT	45.0	45.5	57.9	58.4	943-1158 bp	1.0	72.5
petA-psbJ_p3	TGGGATCTGTTGTTTTGGCA	TCCGGATTAGGTTCATCTCTG	45.0	47.6	57.9	56.6	943-1158 bp	1.0	72.3
petA-psbJ_p4	GGGATCTGTTGTTTTGGCAC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	57.6	56.9	941-1156 bp	1.0	71.6
petA-psbJ_p5	TGGGATCTGTTGTTTTGGCA	TTCCGGATTAGGTTCATCTCTG	45.0	45.5	57.9	57.3	944-1159 bp	1.0	70.0