Hotspot

rbcL-accD

LSC · 54932-56053 · 1122 bp

Back to Plantago

Pi 0.201

MarkerSeek score 74.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 668
pi: 0.201
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	ACACCAGGTCTTCGTCCATA	50.0	50.0	58.0	58.1	1319-1507 bp	0.297872	63.3
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	CAATTGACTAACCTCCCCCG	50.0	55.0	58.0	58.0	1929-2056 bp	0.148936	56.1
rbcL-accD_p3	ACTACAGTTCGGTGGAGGAA	ACTAACCTCCCCCGTGTTTA	50.0	50.0	58.0	58.0	1923-1960 bp	0.06383	45.2
rbcL-accD_p4	GGAGTCAGGAATTAGCTGCC	ACTAACCTCCCCCGTGTTTA	55.0	50.0	58.0	58.0	1768-1805 bp	0.06383	45.1
rbcL-accD_p5	AATGCTACTGCGGGTACATG	ACTAACCTCCCCCGTGTTTA	50.0	50.0	58.0	58.0	2399-2436 bp	0.06383	44.3