Hotspot

trnT-UGU-trnL-UAA

LSC · 45498-46209 · 712 bp

Back to Plantago

Pi 0.0521

MarkerSeek score 78.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 201
pi: 0.0521
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGAAGCCATACCCAATCGAA	ACGCAGTCAACTCCATTTGT	50.0	45.0	58.1	58.0	1592-1780 bp	0.93617	88.8
trnT-UGU-trnL-UAA_p2	TTGACCTGTTGAACCCTTGG	ACGCAGTCAACTCCATTTGT	50.0	45.0	57.9	58.0	1529-1717 bp	0.93617	88.7
trnT-UGU-trnL-UAA_p3	CGAAGCCATACCCAATCGAA	AGTCATTTCCGCCCCAATAC	50.0	50.0	58.1	57.9	2080-2256 bp	0.319149	63.9
trnT-UGU-trnL-UAA_p4	CGAAGCCATACCCAATCGAA	AAAGTCATTTCCGCCCCAAT	50.0	45.0	58.1	58.1	2082-2258 bp	0.319149	63.8
trnT-UGU-trnL-UAA_p5	TTGACCTGTTGAACCCTTGG	AGTCATTTCCGCCCCAATAC	50.0	50.0	57.9	57.9	2017-2193 bp	0.319149	63.8