Hotspot

trnT-GGU-psbD

LSC · 31150-32191 · 1042 bp

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Pi 0.0457

MarkerSeek score 77.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 264
pi: 0.0457
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CATAGTACCCTACCCCCAGG	AGCTCGAATTGGCGTAACAT	60.0	45.0	58.0	58.0	2137-2698 bp	0.978723	90.8
trnT-GGU-psbD_p2	CATAGTACCCTACCCCCAGG	AAAGGTTGTACCCGTGAACC	60.0	50.0	58.0	58.0	1904-2465 bp	0.978723	90.7
trnT-GGU-psbD_p3	CATAGTACCCTACCCCCAGG	CCAGACTGCCCTAGTGGATA	60.0	55.0	58.0	57.9	2239-2800 bp	0.978723	90.2
trnT-GGU-psbD_p4	CATAGTACCCTACCCCCAGG	GCTCGAATTGGCGTAACATG	60.0	50.0	58.0	57.9	2136-2697 bp	0.978723	90.2
trnT-GGU-psbD_p5	CATAGTACCCTACCCCCAGG	AAAGCGCGGAATGTATTTGC	60.0	45.0	58.0	58.1	2446-2993 bp	0.957447	89.4