Hotspot

rbcL

LSC · 56326-57753 · 1428 bp

Back to Plagiopetalum

Pi 0.0007

MarkerSeek score 54.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 1
pi: 0.0007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAGTAGACCTTGTTGCTGT	TGAAGTACGAGAACGAATGCT	50.0	42.9	57.6	57.4	1520 bp	1.0	64.0
rbcL_p2	TCGAGTAGACCTTGTTGCTG	TGAAGTACGAGAACGAATGCT	50.0	42.9	57.3	57.4	1521 bp	1.0	61.7
rbcL_p3	CGAGTAGACCTTGTTGCTGT	TGCAATTGAAGTACGAGAACG	50.0	42.9	57.6	56.9	1526 bp	1.0	60.1
rbcL_p4	CGAGTAGACCTTGTTGCTGT	TGAAGTACGAGAACGAATGC	50.0	45.0	57.6	55.9	1520 bp	1.0	59.9
rbcL_p5	CGAGTAGACCTTGTTGCTGT	TTGAAGTACGAGAACGAATGCT	50.0	40.9	57.6	58.1	1521 bp	1.0	59.8