Hotspot

ndhF-rpl32

SSC · 114871-115696 · 826 bp

Back to Plagiopetalum

Pi 0.00726
MarkerSeek score 51.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
826
variable_sites
6
pi
0.00726
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGAGAGGTATAATCCATGAATAGGG TGAAAAAGCCTTCAATGCTGT 40.0 38.1 57.3 56.9 965-1003 bp 1.0 40.5
ndhF-rpl32_p2 GGAATGAGAGGTATAATCCATGA TGAAAAAGCCTTCAATGCTGT 39.1 38.1 55.1 56.9 969-1007 bp 1.0 40.5
ndhF-rpl32_p3 GAGAGGTATAATCCATGAATAGGGA TGAAAAAGCCTTCAATGCTGT 40.0 38.1 57.0 56.9 964-1002 bp 1.0 40.5
ndhF-rpl32_p4 AGAGGTATAATCCATGAATAGGGA TGAAAAAGCCTTCAATGCTGT 37.5 38.1 55.9 56.9 963-1001 bp 1.0 40.5
ndhF-rpl32_p5 GGAATGAGAGGTATAATCCATGAA TGAAAAAGCCTTCAATGCTGT 37.5 38.1 55.8 56.9 969-1007 bp 1.0 40.5