Hotspot

rpl33-rps18

LSC · 69121-69340 · 220 bp

Back to Plagiopetalum

Pi 0.0273

MarkerSeek score 68.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 6
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGAGTCGCTTGGAATTGAGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.0	57.7	380 bp	1.0	79.2
rpl33-rps18_p2	CGAGTCGCTTGGAATTGAGA	ATTCGATCCCCCGATTGGAT	50.0	50.0	58.0	58.6	377 bp	1.0	76.1
rpl33-rps18_p3	AATCGACACAATACGCCGAG	TCAATTCGATCCCCCGATTG	50.0	50.0	58.4	57.7	396 bp	1.0	75.6
rpl33-rps18_p4	AGAATCGACACAATACGCCG	TCAATTCGATCCCCCGATTG	50.0	50.0	58.4	57.7	398 bp	1.0	75.6
rpl33-rps18_p5	GAATCGACACAATACGCCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.4	57.7	397 bp	1.0	75.6