Hotspot

psbE-petL

LSC · 65789-66926 · 1138 bp

Back to Plagiopetalum

Pi 0.00351

MarkerSeek score 60.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 4
pi: 0.00351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	1224-1225 bp	1.0	51.7
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GCTGAGGTTATAGTTAAAGCTGC	45.0	43.5	58.2	57.6	1310-1311 bp	1.0	51.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.2	57.6	1315-1316 bp	1.0	51.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	1219-1220 bp	1.0	51.4
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	1217-1218 bp	1.0	50.2