Hotspot

rbcL-accD

LSC · 57754-58371 · 618 bp

Back to Plagiopetalum

Pi 0.00971

MarkerSeek score 64.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 6
pi: 0.00971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	706-707 bp	1.0	77.6
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ATCCTCACTTCCACGAGTGT	50.0	50.0	57.9	58.4	795-796 bp	1.0	76.8
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACATCGAATTAAACCGCCGT	55.0	45.0	57.7	58.3	722-723 bp	1.0	76.2
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CACTTCCACGAGTGTCTTCA	50.0	50.0	57.9	57.5	790-791 bp	1.0	75.6
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ATCCTCACTTCCACGAGTGT	55.0	50.0	57.7	58.4	811-812 bp	1.0	75.3