Hotspot

ndhC-trnV-UAC

LSC · 51708-52445 · 738 bp

Back to Plagiopetalum

Pi 0.0041

MarkerSeek score 62.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 3
pi: 0.0041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCGGAAATTCGAAATGCCA	GGTTCGAATCCGTATAGCCC	45.0	55.0	58.2	57.9	840-847 bp	1.0	79.0
ndhC-trnV-UAC_p2	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.7	57.9	866-873 bp	1.0	78.3
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	855-862 bp	1.0	77.8
ndhC-trnV-UAC_p4	TCCGGAAATTCGAAATGCCA	CGGTTCGAATCCGTATAGCC	45.0	55.0	58.2	58.3	841-848 bp	1.0	77.1
ndhC-trnV-UAC_p5	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.7	58.3	867-874 bp	1.0	76.5