Hotspot

trnD-GUC-trnY-GUA

LSC · 30806-31279 · 474 bp

Back to Plagiopetalum

Pi 0.00219
MarkerSeek score 53.80
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
474
variable_sites
1
pi
0.00219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 528-545 bp 1.0 76.4
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 527-544 bp 1.0 76.4
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GAATGGCTTCTTCCTGGGTC 50.0 55.0 58.6 58.2 587-604 bp 1.0 76.1
trnD-GUC-trnY-GUA_p4 TTTATTCCGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 581-598 bp 1.0 75.2
trnD-GUC-trnY-GUA_p5 TTTATTCCGTCGGGACTGAC GGCAATATGTCTACGCTGGT 50.0 50.0 57.3 57.8 580-597 bp 1.0 75.2