Hotspot

trnS-GCU-trnR-UCU

LSC · 7949-9770 · 1822 bp

Back to Plagiopetalum

Pi 0.00659

MarkerSeek score 61.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1822
variable_sites: 12
pi: 0.00659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1936-1961 bp	1.0	68.9
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1935-1960 bp	1.0	68.9
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1943-1968 bp	1.0	67.0
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1942-1967 bp	1.0	67.0
trnS-GCU-trnR-UCU_p5	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1937-1962 bp	1.0	66.9