Hotspot

rps16-trnQ-UUG

LSC · 5704-6712 · 1009 bp

Back to Plagiopetalum

Pi 0.00303
MarkerSeek score 50.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1009
variable_sites
3
pi
0.00303
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1058-1074 bp 1.0 74.8
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1061-1077 bp 1.0 72.6
rps16-trnQ-UUG_p3 TAAGAATCCACGGACGATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 56.8 58.1 1094-1110 bp 1.0 71.7
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 1062-1078 bp 1.0 70.1
rps16-trnQ-UUG_p5 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 1060-1076 bp 1.0 69.5