Hotspot

ycf1

Genome · 107656-112464 · 4809 bp

Back to Plagiomnium

Pi 0.000416

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4809
variable_sites: 2
pi: 0.000416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACGCATTTTAGCAGAAAGTGA	TGGTTAATTCCAGCATTTATGGA	40.9	34.8	57.9	56.2	271 bp	1.0	40.6
ycf1_p2	TCACGCATTTTAGCAGAAAGTG	TGGTTAATTCCAGCATTTATGGA	40.9	34.8	57.9	56.2	272 bp	1.0	40.6
ycf1_p3	CACGCATTTTAGCAGAAAGTGA	AGTTCTATGGTTAATTCCAGCA	40.9	36.4	57.9	55.1	278 bp	1.0	40.6
ycf1_p4	TCACGCATTTTAGCAGAAAGTG	AGTTCTATGGTTAATTCCAGCA	40.9	36.4	57.9	55.1	279 bp	1.0	40.6
ycf1_p5	ACGCATTTTAGCAGAAAGTGA	TGGTTAATTCCAGCATTTATGGA	38.1	34.8	56.5	56.2	270 bp	1.0	40.6