Hotspot

psbC

Genome · 43416-44837 · 1422 bp

Back to Plagiomnium

Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGTCGAGCCATTTTAAAATATCA	AGGAAATTCGTGCAGCAGAA	30.4	45.0	54.4	58.1	1573 bp	1.0	40.1
psbC_p2	AGTCGAGCCATTTTAAAATATCA	GGAAATTCGTGCAGCAGAAG	30.4	50.0	54.4	57.7	1572 bp	1.0	40.1
psbC_p3	AGTCGAGCCATTTTAAAATATCA	GCAGCAGAAGATCCGGAATT	30.4	50.0	54.4	58.3	1562 bp	1.0	40.1
psbC_p4	AGTCGAGCCATTTTAAAATATCA	ATGAAGGTATCCGTGCTTGG	30.4	50.0	54.4	57.7	1510 bp	1.0	40.1
psbC_p5	AGTCGAGCCATTTTAAAATATCA	ATTCGTGCAGCAGAAGATCC	30.4	50.0	54.4	58.3	1568 bp	1.0	40.1