Hotspot

psaA

Genome · 37137-39389 · 2253 bp

Back to Plagiomnium

Pi 0.000444

MarkerSeek score 57.40

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: Genome
length_bp: 2253
variable_sites: 1
pi: 0.000444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	TCCTATGCCTCGTCTGAAGA	GCGAGTAGTTGGGTCTTGAG	50.0	55.0	57.6	58.0	2368 bp	1.0	76.5
psaA_p2	TCCTATGCCTCGTCTGAAGA	CAGATACGGCGAGTAGTTGG	50.0	55.0	57.6	57.9	2376 bp	1.0	75.6
psaA_p3	TCCTATGCCTCGTCTGAAGA	CCAGATACGGCGAGTAGTTG	50.0	55.0	57.6	57.9	2377 bp	1.0	75.6
psaA_p4	TCCTATGCCTCGTCTGAAGA	TAGGCCCTGGCTGAATTTTG	50.0	50.0	57.6	58.1	2347 bp	1.0	75.3
psaA_p5	TCCTATGCCTCGTCTGAAGA	ACCAGATACGGCGAGTAGTT	50.0	50.0	57.6	58.2	2378 bp	1.0	74.6