Hotspot

petD

Genome · 7441-8561 · 1121 bp

Back to Plagiomnium

Pi 0.000892

MarkerSeek score 59.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: Genome
length_bp: 1121
variable_sites: 1
pi: 0.000892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTTGCGCAAAGGTAAACG	GGTCGGCAAGTATGATGGTT	45.0	50.0	57.9	58.0	2322 bp	1.0	78.8
petD_p2	GCGGACAAGTTGTTTCTTGG	GGTCGGCAAGTATGATGGTT	50.0	50.0	57.9	58.0	2231 bp	1.0	78.8
petD_p3	TCCAGGACGAGATACAGCTT	GGTCGGCAAGTATGATGGTT	50.0	50.0	57.9	58.0	2074 bp	1.0	78.6
petD_p4	AGTTTGCGCAAAGGTAAACG	ACCTCTCTTAACTGCGGTCT	45.0	50.0	57.9	58.1	2012 bp	1.0	78.5
petD_p5	TGCGCAAAGGTAAACGTAGA	GGTCGGCAAGTATGATGGTT	45.0	50.0	57.8	58.0	2318 bp	1.0	78.5