Hotspot

ndhF

Genome · 96494-98644 · 2151 bp

Back to Plagiomnium

Pi 0.000465

MarkerSeek score 57.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2151
variable_sites: 1
pi: 0.000465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGCGATCGTACCATCATTA	AGAATCCTAAACTTTTTCTTTGT	50.0	26.1	57.7	52.5	2348 bp	1.0	40.0
ndhF_p2	GTAGTGGGAAGTGAGGGGAA	AGAATCCTAAACTTTTTCTTTGT	55.0	26.1	58.3	52.5	2375 bp	1.0	40.0
ndhF_p3	TGGGAAGTGAGGGGAATACA	AGAATCCTAAACTTTTTCTTTGT	50.0	26.1	57.6	52.5	2371 bp	1.0	40.0
ndhF_p4	CCGCGATCGTACCATCATTA	GGATAGAATCCTAAACTTTTTCTTTGT	50.0	29.6	57.7	56.4	2352 bp	1.0	40.0
ndhF_p5	GTAGTGGGAAGTGAGGGGAA	GGATAGAATCCTAAACTTTTTCTTTGT	55.0	29.6	58.3	56.4	2379 bp	1.0	40.0