Hotspot

atpI

Genome · 68095-68841 · 747 bp

Back to Plagiomnium

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 747
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TTGCAAATGAAAGCGCCAAA	GATGCTCGAGCTTCTATCCG	40.0	55.0	58.1	58.0	1705 bp	1.0	79.4
atpI_p2	TTGCAAATGAAAGCGCCAAA	GCTCGAGCTTCTATCCGATG	40.0	55.0	58.1	58.0	1702 bp	1.0	79.4
atpI_p3	TTTGCAAATGAAAGCGCCAA	GATGCTCGAGCTTCTATCCG	40.0	55.0	58.1	58.0	1706 bp	1.0	79.4
atpI_p4	TTTGCAAATGAAAGCGCCAA	GCTCGAGCTTCTATCCGATG	40.0	55.0	58.1	58.0	1703 bp	1.0	79.4
atpI_p5	TTGCAAATGAAAGCGCCAAA	GCAAATGATGATGCTCGAGC	40.0	50.0	58.1	58.0	1714 bp	1.0	79.3