Hotspot

rbcL-atpB

Genome · 27565-28123 · 559 bp

Back to Plagiomnium

Pi 0.00179

MarkerSeek score 58.90

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 559
variable_sites: 1
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GTCTCCGTTTGTGGTGACAT	GGCAATAAGTGTTGAAGTCCC	50.0	47.6	58.1	57.1	624 bp	1.0	64.4
rbcL-atpB_p2	GTCTCCGTTTGTGGTGACAT	ACGTCCTACATTTTTGGCAA	50.0	40.0	58.1	55.9	639 bp	1.0	62.0
rbcL-atpB_p3	GTCTCCGTTTGTGGTGACAT	TGTTGAAGTCCCAAAAGTACGA	50.0	40.9	58.1	57.9	615 bp	1.0	62.0
rbcL-atpB_p4	GTCTCCGTTTGTGGTGACAT	AGGCATTTTACCTGGAGAGA	50.0	45.0	58.1	55.8	696 bp	1.0	61.7
rbcL-atpB_p5	CACCTGCTTTAGTCTCCGTT	GGCAATAAGTGTTGAAGTCCC	50.0	47.6	57.5	57.1	635 bp	1.0	61.7