Hotspot

psaI-accD

Genome · 24479-24755 · 277 bp

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Pi 0
MarkerSeek score 26.70
Primer available yes
Label psaI-accD

Feature Metadata

label_name
psaI-accD
feature_type
igs
region
Genome
length_bp
277
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-accD_p1 AAGCCATTGTAATCGCTGGA ACTTGATTTAATTGTCCCACGT 45.0 36.4 57.6 56.3 456 bp 1.0 46.8
psaI-accD_p2 AGCCATTGTAATCGCTGGAA ACTTGATTTAATTGTCCCACGT 45.0 36.4 57.6 56.3 455 bp 1.0 46.8
psaI-accD_p3 GAAGCCATTGTAATCGCTGG ACTTGATTTAATTGTCCCACGT 50.0 36.4 57.2 56.3 457 bp 1.0 44.0
psaI-accD_p4 AGCCATTGTAATCGCTGGAA ACTTGATTTAATTGTCCCACG 45.0 38.1 57.6 54.5 455 bp 1.0 40.5
psaI-accD_p5 AGCCATTGTAATCGCTGGAA CTTGATTTAATTGTCCCACGT 45.0 38.1 57.6 54.5 454 bp 1.0 40.5