Hotspot

rrn23

Genome · 154374-157257 · 2884 bp

Back to Plagiogyria

Pi 0.0323
MarkerSeek score 76.70
Primer available yes
Label rrn23

Feature Metadata

label_name
rrn23
feature_type
rRNA
region
Genome
length_bp
2884
variable_sites
91
pi
0.0323
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23_p1 ACTTCTGTCGTTTCACGCTT TCGTAGCATTCAACGACGAG 45.0 50.0 58.1 58.1 2927-2994 bp 1.0 83.0
rrn23_p2 TTCTGCGTCTAGAGTCTCCC TCGTAGCATTCAACGACGAG 55.0 50.0 58.2 58.1 2872-2939 bp 1.0 81.6
rrn23_p3 CGGCTTTTTCTGCGTCTAGA TCGTAGCATTCAACGACGAG 50.0 50.0 58.3 58.1 2879-2946 bp 1.0 81.2
rrn23_p4 TTCTGCGTCTAGAGTCTCCC AGCACGTTGAAGTGTCGTAG 55.0 50.0 58.2 58.2 2886-2953 bp 1.0 80.6
rrn23_p5 TTCTGCGTCTAGAGTCTCCC AGTAGCACGTTGAAGTGTCG 55.0 50.0 58.2 58.2 2889-2956 bp 1.0 80.6
rrn23_p1 TCGTAGCATTCAACGACGAG ACTTCTGTCGTTTCACGCTT 50.0 45.0 58.1 58.1 2927-2994 bp 1.0 83.0
rrn23_p2 TCGTAGCATTCAACGACGAG TTCTGCGTCTAGAGTCTCCC 50.0 55.0 58.1 58.2 2872-2939 bp 1.0 81.6
rrn23_p3 TCGTAGCATTCAACGACGAG CGGCTTTTTCTGCGTCTAGA 50.0 50.0 58.1 58.3 2879-2946 bp 1.0 81.2
rrn23_p4 AGCACGTTGAAGTGTCGTAG TTCTGCGTCTAGAGTCTCCC 50.0 55.0 58.2 58.2 2886-2953 bp 1.0 80.6
rrn23_p5 AGTAGCACGTTGAAGTGTCG TTCTGCGTCTAGAGTCTCCC 50.0 55.0 58.2 58.2 2889-2956 bp 1.0 80.6