Hotspot

rpl21-rpl32

Genome · 118134-118326 · 193 bp

Back to Plagiogyria

Pi 0.0207

MarkerSeek score 64.40

Primer available yes

Label rpl21-rpl32

Feature Metadata

label_name: rpl21-rpl32
feature_type: igs
region: Genome
length_bp: 193
variable_sites: 4
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-rpl32_p1	AATTTGGTCCGATTCGTGGT	GCTACTTTCACCGTTTTGGC	45.0	50.0	57.8	58.0	339-345 bp	1.0	79.0
rpl21-rpl32_p2	ATTTGGTCCGATTCGTGGTC	GCTACTTTCACCGTTTTGGC	50.0	50.0	58.3	58.0	338-344 bp	1.0	78.4
rpl21-rpl32_p3	AATTTGGTCCGATTCGTGGT	GGCCGAGGAAAAAGCTTTTG	45.0	50.0	57.8	58.2	361-367 bp	1.0	77.6
rpl21-rpl32_p4	AATTTGGTCCGATTCGTGGT	CGTTTTGGCCCTCCATACAT	45.0	50.0	57.8	58.2	328-334 bp	1.0	77.5
rpl21-rpl32_p5	ATTTGGTCCGATTCGTGGTC	GGCCGAGGAAAAAGCTTTTG	50.0	50.0	58.3	58.2	360-366 bp	1.0	77.0