Hotspot

psbT-psbN

Genome · 78505-78621 · 117 bp

Back to Plagiogyria

Pi 0.0522

MarkerSeek score 82.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 117
variable_sites: 6
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCACCCAAAGTTC	AGAGACCCTTTCGAGGAACA	50.0	50.0	57.8	58.0	182-184 bp	1.0	81.4
psbT-psbN_p2	CCCATGGAAGCACTGGTTTA	AGAGACCCTTTCGAGGAACA	50.0	50.0	57.8	58.0	253-255 bp	1.0	80.7
psbT-psbN_p3	TCTACGGTTGAACCCATGGA	AGAGACCCTTTCGAGGAACA	50.0	50.0	58.4	58.0	265-267 bp	1.0	79.5
psbT-psbN_p4	CCCATGGAAGCACTGGTTTA	CTCAGAGACCCTTTCGAGGA	50.0	55.0	57.8	58.2	256-258 bp	1.0	79.5
psbT-psbN_p5	GTTGAACCCATGGAAGCACT	AGAGACCCTTTCGAGGAACA	50.0	50.0	58.4	58.0	259-261 bp	1.0	79.3