Hotspot

rpl33-rps18

Genome · 71775-71833 · 59 bp

Back to Plagiogyria

Pi 0.0339

MarkerSeek score 67.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: Genome
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGCACACCAACCACAAAG	GAACGTCTACGGGAAGATCG	50.0	55.0	58.1	57.9	128 bp	1.0	79.8
rpl33-rps18_p2	CAAGCACACCAACCACAAAG	TCTTCCCTGCTCACTTACGA	50.0	50.0	58.1	58.1	207 bp	1.0	79.5
rpl33-rps18_p3	ACTACGCGTAAGAATCGTCG	TCTTCCCTGCTCACTTACGA	50.0	50.0	57.9	58.1	275 bp	1.0	79.3
rpl33-rps18_p4	CTACGCGTAAGAATCGTCGT	TCTTCCCTGCTCACTTACGA	50.0	50.0	57.9	58.1	274 bp	1.0	79.3
rpl33-rps18_p5	CTCGGTACACTACGCGTAAG	TCTTCCCTGCTCACTTACGA	55.0	50.0	57.9	58.1	283 bp	1.0	79.3