Hotspot

psbD-trnT-GGU

Genome · 41328-42882 · 1555 bp

Back to Plagiogyria

Pi 0.0103

MarkerSeek score 64.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1555
variable_sites: 16
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTTCTTAGCCAGTCGTCCA	TAAGCCGTCGGTTCAAATCC	50.0	50.0	58.1	58.3	1661-1665 bp	1.0	78.0
psbD-trnT-GGU_p2	TCTTCTTAGCCAGTCGTCCA	GGCCCTTTTAACTCAGTGGT	50.0	50.0	58.1	57.7	1703-1707 bp	1.0	77.8
psbD-trnT-GGU_p3	CGGACCAACCTACGAAAACA	TAAGCCGTCGGTTCAAATCC	50.0	50.0	58.1	58.3	1689-1693 bp	1.0	77.5
psbD-trnT-GGU_p4	CGGACCAACCTACGAAAACA	GGCCCTTTTAACTCAGTGGT	50.0	50.0	58.1	57.7	1731-1735 bp	1.0	77.4
psbD-trnT-GGU_p5	TCTTCTTAGCCAGTCGTCCA	AGGCCCTTTTAACTCAGTGG	50.0	50.0	58.1	57.4	1704-1708 bp	1.0	75.4