Hotspot

trnE-UUC-trnY-GUA

Genome · 27705-27857 · 153 bp

Back to Plagiogyria

Pi 0.0196
MarkerSeek score 63.80
Primer available yes
Label trnE-UUC-trnY-GUA

Feature Metadata

label_name
trnE-UUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
153
variable_sites
3
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnY-GUA_p1 CTCTCTTTCAAGGAGGCGAC CACGATGCCACAAACGAATC 55.0 50.0 58.0 58.1 314 bp 1.0 79.8
trnE-UUC-trnY-GUA_p2 CTCTCTTTCAAGGAGGCGAC CGATGCCACAAACGAATCTG 55.0 50.0 58.0 57.8 312 bp 1.0 79.4
trnE-UUC-trnY-GUA_p3 CTCTCTTTCAAGGAGGCGAC ACGATGCCACAAACGAATCT 55.0 45.0 58.0 58.2 313 bp 1.0 79.2
trnE-UUC-trnY-GUA_p4 CACCTCTCTTTCAAGGAGGC CACGATGCCACAAACGAATC 55.0 50.0 57.9 58.1 317 bp 1.0 79.0
trnE-UUC-trnY-GUA_p5 CACCTCTCTTTCAAGGAGGC CGATGCCACAAACGAATCTG 55.0 50.0 57.9 57.8 315 bp 1.0 78.6