Hotspot

rpoC1

LSC · 20717-23596 · 2880 bp

Back to Pittosporum

Pi 0.00106

MarkerSeek score 55.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2880
variable_sites: 14
pi: 0.00106
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCCGGAATTCTCGTCCTAGT	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	2984-2998 bp	1.0	76.5
rpoC1_p2	TCCGGAATTCTCGTCCTAGT	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.6	57.6	2983-2997 bp	1.0	74.1
rpoC1_p3	CGTCCTAGTTGAACCGGAGA	ACGATCTTTGGCTCTGGAAC	55.0	50.0	58.8	57.9	2973-2987 bp	1.0	73.3
rpoC1_p4	CTCGTCCTAGTTGAACCGGA	ACGATCTTTGGCTCTGGAAC	55.0	50.0	58.8	57.9	2975-2989 bp	1.0	73.3
rpoC1_p5	TCGTCCTAGTTGAACCGGAG	ACGATCTTTGGCTCTGGAAC	55.0	50.0	58.8	57.9	2974-2988 bp	1.0	73.3