Hotspot

ndhD-psaC

SSC · 117043-117164 · 122 bp

Back to Pittosporum

Pi 0.0194

MarkerSeek score 56.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 122
variable_sites: 10
pi: 0.0194
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAGCCCGCGAATATTGGTAA	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.1	58.0	290 bp	1.0	82.0
ndhD-psaC_p2	AGCCCGCGAATATTGGTAAA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.7	58.0	289 bp	1.0	80.3
ndhD-psaC_p3	ATAGAGCCCGCGAATATTGG	AGAGGACTGTGTCGGTTGTA	50.0	50.0	57.6	58.0	293 bp	1.0	79.8
ndhD-psaC_p4	GAGCCCGCGAATATTGGTAA	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.1	57.7	271 bp	1.0	79.7
ndhD-psaC_p5	GAGCCCGCGAATATTGGTAA	GAGATGTGAATCCGCCTGTC	50.0	55.0	58.1	58.4	269 bp	1.0	78.9