Hotspot

ndhF-trnL-UAG

SSC · 111953-114155 · 2203 bp

Back to Pittosporum

Pi 0.00275
MarkerSeek score 57.80
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
2203
variable_sites
34
pi
0.00275
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CTGGAAGTGGAAGGAAAGGT AGCGTGTCTACCGATTTCAC 50.0 50.0 57.0 58.0 2159-2314 bp 1.0 77.5
ndhF-trnL-UAG_p2 CTGGAAGTGGAAGGAAAGGT CAGCGTGTCTACCGATTTCA 50.0 50.0 57.0 58.0 2160-2315 bp 1.0 77.4
ndhF-trnL-UAG_p3 CTGGAAGTGGAAGGAAAGGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.0 57.9 2169-2324 bp 1.0 76.5
ndhF-trnL-UAG_p4 CTGGAAGTGGAAGGAAAGGT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.0 57.8 2168-2323 bp 1.0 75.9
ndhF-trnL-UAG_p5 CTGGAAGTGGAAGGAAAGGT CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.0 57.8 2167-2322 bp 1.0 75.9