Hotspot

petD-rps11

LSC · 78573-79830 · 1258 bp

Back to Pittosporum

Pi 0.0017

MarkerSeek score 55.10

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: LSC
length_bp: 1258
variable_sites: 13
pi: 0.0017
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	TTGGTTGGGTATTGGTGCAA	AAGAGACGCAGCATTACGAG	45.0	50.0	57.9	58.1	1428-1437 bp	1.0	79.7
petD-rps11_p2	TTTGGTTGGGTATTGGTGCA	AAGAGACGCAGCATTACGAG	45.0	50.0	57.9	58.1	1429-1438 bp	1.0	79.7
petD-rps11_p3	TTGGTTGGGTATTGGTGCAA	GAAGAGACGCAGCATTACGA	45.0	50.0	57.9	58.1	1429-1438 bp	1.0	79.6
petD-rps11_p4	TTTGGTTGGGTATTGGTGCA	GAAGAGACGCAGCATTACGA	45.0	50.0	57.9	58.1	1430-1439 bp	1.0	79.6
petD-rps11_p5	TTGGTTGGGTATTGGTGCAA	AACTTTCGTGCGGGATGTAA	45.0	45.0	57.9	57.8	1383-1392 bp	1.0	79.0