Hotspot

petB-petD

LSC · 77165-78047 · 883 bp

Back to Pittosporum

Pi 0.00362
MarkerSeek score 46.70
Primer available yes
Label petB-petD

Feature Metadata

label_name
petB-petD
feature_type
igs
region
LSC
length_bp
883
variable_sites
9
pi
0.00362
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB-petD_p1 CCGCTTCTTACTGCCGTATT TGACCCATACCTTTAGCCAA 50.0 45.0 58.1 56.1 1071-1072 bp 1.0 65.1
petB-petD_p2 CCGCTTCTTACTGCCGTATT ATGACCCATACCTTTAGCCA 50.0 45.0 58.1 55.9 1072-1073 bp 1.0 63.2
petB-petD_p3 CCGCTTCTTACTGCCGTATT TCTTATAGATCCATCCCCCGA 50.0 47.6 58.1 56.9 992-993 bp 1.0 63.2
petB-petD_p4 TATTGCCGCTTCTTACTGCC TGACCCATACCTTTAGCCAA 50.0 45.0 58.3 56.1 1076-1077 bp 1.0 62.9
petB-petD_p5 ACACACTTTTGTATTGCCGC TGACCCATACCTTTAGCCAA 45.0 45.0 57.6 56.1 1087-1088 bp 1.0 62.3