Hotspot

rpl33-rps18

LSC · 68765-68937 · 173 bp

Back to Pittosporum

Pi 0.0139

MarkerSeek score 58.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 18
pi: 0.0139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGCACAATACGCCTAATCGA	CAATTCGATCCCCCGATTGT	50.0	50.0	58.1	58.0	344 bp	1.0	83.3
rpl33-rps18_p2	ACTCAAAAGAACCGGCACAA	CAATTCGATCCCCCGATTGT	45.0	50.0	58.2	58.0	357 bp	1.0	82.3
rpl33-rps18_p3	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	345 bp	1.0	81.5
rpl33-rps18_p4	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	358 bp	1.0	80.5
rpl33-rps18_p5	TACTCAAAAGAACCGGCACA	CAATTCGATCCCCCGATTGT	45.0	50.0	57.4	58.0	358 bp	1.0	79.2