Hotspot

psbE-petL

LSC · 65574-66769 · 1196 bp

Back to Pittosporum

Pi 0.0035

MarkerSeek score 62.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 19
pi: 0.0035
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1044-1264 bp	1.0	84.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1046-1266 bp	1.0	83.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1130-1350 bp	1.0	83.8
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.2	58.0	1132-1352 bp	1.0	83.7
psbE-petL_p5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.2	58.0	1135-1355 bp	1.0	83.7