Hotspot

accD-psaI

LSC · 59726-60433 · 708 bp

Back to Pittosporum

Pi 0.00336

MarkerSeek score 56.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 17
pi: 0.00336
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	822-835 bp	1.0	77.7
accD-psaI_p2	ACTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	821-834 bp	1.0	77.7
accD-psaI_p3	AACTCCACGCTTTCTTTCCT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	812-825 bp	1.0	77.4
accD-psaI_p4	ACTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	811-824 bp	1.0	77.4
accD-psaI_p5	CAACTCCACGCTTTCTTTCC	AAAGAAGCCATTGCCATTGC	50.0	45.0	57.3	57.9	823-836 bp	1.0	77.3