Hotspot

ndhC-trnV-UAC

LSC · 51260-52402 · 1143 bp

Back to Pittosporum

Pi 0.00415
MarkerSeek score 58.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1143
variable_sites
22
pi
0.00415
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGGCTAAAACTACGGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1266-1283 bp 1.0 80.2
ndhC-trnV-UAC_p2 CGGGGCTAAAACTACGGAAA AACGCATGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 1333-1350 bp 1.0 79.2
ndhC-trnV-UAC_p3 CGGGGCTAAAACTACGGAAA ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 1332-1349 bp 1.0 79.2
ndhC-trnV-UAC_p4 AATCGGGGCTAAAACTACGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1269-1286 bp 1.0 78.4
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1277-1294 bp 0.933333 75.4