Hotspot

trnT-CGU-psbD

LSC · 32076-33468 · 1393 bp

Back to Pittosporum

Pi 0.0019

MarkerSeek score 57.60

Primer available yes

Label trnT-CGU-psbD

Feature Metadata

label_name: trnT-CGU-psbD
feature_type: igs
region: LSC
length_bp: 1393
variable_sites: 16
pi: 0.0019
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-CGU-psbD_p1	AGGCCCTTTTAACTCAACGG	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1566-1594 bp	1.0	79.4
trnT-CGU-psbD_p2	CTCAACGGTAGAGTAACGCC	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1554-1582 bp	1.0	79.4
trnT-CGU-psbD_p3	AGGCCCTTTTAACTCAACGG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1556-1584 bp	1.0	78.4
trnT-CGU-psbD_p4	CTCAACGGTAGAGTAACGCC	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1544-1572 bp	1.0	78.4
trnT-CGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1527-1555 bp	1.0	77.9