Hotspot

trnE-UUC-trnT-CGU

LSC · 31286-32003 · 718 bp

Back to Pittosporum

Pi 0.00333

MarkerSeek score 61.80

Primer available yes

Label trnE-UUC-trnT-CGU

Feature Metadata

label_name: trnE-UUC-trnT-CGU
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 10
pi: 0.00333
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-CGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	813-833 bp	1.0	83.7
trnE-UUC-trnT-CGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	850-870 bp	1.0	83.6
trnE-UUC-trnT-CGU_p3	TTTCGTAGTACCCTACCCCC	GGCGTTACTCTACCGTTGAG	55.0	55.0	57.8	58.1	825-845 bp	1.0	82.9
trnE-UUC-trnT-CGU_p4	CGTTGCCTCCTTGAAAGAGA	GGCGTTACTCTACCGTTGAG	50.0	55.0	57.8	58.1	788-808 bp	1.0	82.9
trnE-UUC-trnT-CGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	791-811 bp	1.0	82.8