Hotspot

trnS-GCU-trnT-CGU

LSC · 8370-9130 · 761 bp

Back to Pittosporum

Pi 0.00403

MarkerSeek score 53.80

Primer available yes

Label trnS-GCU-trnT-CGU

Feature Metadata

label_name: trnS-GCU-trnT-CGU
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 16
pi: 0.00403
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnT-CGU_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCACT	45.0	42.9	58.0	58.2	817-855 bp	1.0	73.8
trnS-GCU-trnT-CGU_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCAC	45.0	45.0	58.0	56.8	817-855 bp	1.0	73.8
trnS-GCU-trnT-CGU_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	866-904 bp	1.0	72.7
trnS-GCU-trnT-CGU_p4	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.2	56.8	866-904 bp	1.0	72.6
trnS-GCU-trnT-CGU_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCGCACTTTTACCAC	45.0	42.9	58.0	57.5	818-856 bp	1.0	71.5