Hotspot

clpP

LSC · 78282-80368 · 2087 bp

Back to Pithecellobium

Pi 0.0535

MarkerSeek score 73.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2087
variable_sites: 110
pi: 0.0535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTAGTACATGTTCCTCGGCG	TGGACAGAAAGTAAACGGCC	55.0	50.0	57.9	58.1	2976 bp	0.5	58.1
clpP_p2	CTAGTACATGTTCCTCGGCG	AAACGGCCGGGATCATTTAA	55.0	45.0	57.9	57.9	2964 bp	0.5	58.0
clpP_p3	AGTCGCACATACACCCTAGT	TGGACAGAAAGTAAACGGCC	50.0	50.0	58.2	58.1	2991 bp	0.5	57.9
clpP_p4	AGTCGCACATACACCCTAGT	AAACGGCCGGGATCATTTAA	50.0	45.0	58.2	57.9	2979 bp	0.5	57.7
clpP_p5	TGACATTTCGGATTGGCTGT	TGGACAGAAAGTAAACGGCC	45.0	50.0	57.8	58.1	2924 bp	0.5	57.5