Hotspot

ccsA

SSC · 136691-137662 · 972 bp

Back to Pithecellobium

Pi 0.0237

MarkerSeek score 58.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 23
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1466-1506 bp	1.0	84.1
ccsA_p2	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1465-1505 bp	1.0	84.1
ccsA_p3	GTGAAATCGGTAGACACGCT	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1662-1702 bp	1.0	83.1
ccsA_p4	GTGAAATCGGTAGACACGCT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1748-1788 bp	1.0	83.0
ccsA_p5	TGAAATCGGTAGACACGCTG	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1747-1787 bp	1.0	83.0