Hotspot

ycf1-trnN-GUU

IRa · 152210-152800 · 591 bp

Back to Pithecellobium

Pi 0.0727

MarkerSeek score 79.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 591
variable_sites: 40
pi: 0.0727
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	GCAAAAATGCCTTCCTCGTC	50.0	50.0	58.2	58.0	1053-1078 bp	1.0	85.2
ycf1-trnN-GUU_p2	CTTCTCGGTTGCTTCTTCCA	GCAAAAATGCCTTCCTCGTC	50.0	50.0	57.8	58.0	1137-1162 bp	1.0	84.8
ycf1-trnN-GUU_p3	AAAACAGGCAGTGGTGTTCT	GCAAAAATGCCTTCCTCGTC	45.0	50.0	57.9	58.0	1718-1743 bp	1.0	83.4
ycf1-trnN-GUU_p4	AAAACAGGCAGTGGTGTTCT	CGGGAGAATTGATGACTGCA	45.0	50.0	57.9	58.0	1664-1689 bp	1.0	83.4
ycf1-trnN-GUU_p5	AAAACAGGCAGTGGTGTTCT	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.9	58.0	1755-1780 bp	1.0	83.1