Hotspot

trnL-UAG-ccsA

SSC · 136605-136690 · 86 bp

Back to Pithecellobium

Pi 0.0581

MarkerSeek score 71.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 86
variable_sites: 5
pi: 0.0581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACCGAAAACGATATATGAGTT	50.0	33.3	57.9	52.8	197 bp	1.0	42.0
trnL-UAG-ccsA_p2	GGTAGACACGCTGCTCTTAG	ACCGAAAACGATATATGAGTT	55.0	33.3	57.8	52.8	199 bp	1.0	42.0
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	ACCGAAAACGATATATGAGTT	55.0	33.3	57.8	52.8	198 bp	1.0	42.0
trnL-UAG-ccsA_p4	GTGAAATCGGTAGACACGCT	ACCGAAAACGATATATGAGTT	50.0	33.3	58.0	52.8	207 bp	1.0	41.9
trnL-UAG-ccsA_p5	TGAAATCGGTAGACACGCTG	ACCGAAAACGATATATGAGTT	50.0	33.3	58.0	52.8	206 bp	1.0	41.9