Hotspot

ndhF-rpl32

SSC · 135407-136095 · 689 bp

Back to Pithecellobium

Pi 0.0385

MarkerSeek score 69.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 689
variable_sites: 26
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAGAAAGGCGCACAAAGTA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1844-2098 bp	1.0	82.4
ndhF-rpl32_p2	ACAGAAAGGCGCACAAAGTA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1845-2099 bp	1.0	82.3
ndhF-rpl32_p3	GGGGAATTGGGCAGACTTAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.9	58.0	1882-2136 bp	1.0	82.1
ndhF-rpl32_p4	GGGGAATTGGGCAGACTTAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.9	58.0	1883-2137 bp	1.0	82.1
ndhF-rpl32_p5	AGCAACTGAACCGCCAAATA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	1864-2118 bp	1.0	81.9