Hotspot

psaC-ndhD

IRb · 131179-131305 · 127 bp

Back to Pithecellobium

Pi 0.0551

MarkerSeek score 81.90

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: IRb
length_bp: 127
variable_sites: 7
pi: 0.0551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AAACAACTCGAAGCATGGGT	CCGCGGATATTGGGAAAACT	45.0	50.0	58.0	58.3	211 bp	1.0	79.9
psaC-ndhD_p2	AAACAACTCGAAGCATGGGT	AGAAGATTAAGGAACCCGCG	45.0	50.0	58.0	57.7	226 bp	1.0	79.8
psaC-ndhD_p3	GGCATGAAACAACTCGAAGC	AGAAGATTAAGGAACCCGCG	50.0	50.0	58.0	57.7	232 bp	1.0	79.7
psaC-ndhD_p4	GGCATGAAACAACTCGAAGC	CCGCGGATATTGGGAAAACT	50.0	50.0	58.0	58.3	217 bp	1.0	79.7
psaC-ndhD_p5	AAACAACTCGAAGCATGGGT	TAAGGAACCCGCGGATATTG	45.0	50.0	58.0	57.5	219 bp	1.0	78.1