Hotspot

rps12-clpP

LSC · 78023-78281 · 259 bp

Back to Pithecellobium

Pi 0.0803

MarkerSeek score 74.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 20
pi: 0.0803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CCCTCTTAGTCCGTTCTTGC	AGATGCCTGTACGGTTGTTC	55.0	50.0	58.0	57.9	1050-1141 bp	1.0	85.3
rps12-clpP_p2	GTGAAGATTAGCTGGGGAGC	AGATGCCTGTACGGTTGTTC	55.0	50.0	58.0	57.9	1073-1164 bp	1.0	84.9
rps12-clpP_p3	CCCTCTTAGTCCGTTCTTGC	ACGCCCAAGTTCATGGAATT	55.0	45.0	58.0	58.1	756 bp	0.5	59.3
rps12-clpP_p4	GTGAAGATTAGCTGGGGAGC	ACGCCCAAGTTCATGGAATT	55.0	45.0	58.0	58.1	779 bp	0.5	59.1
rps12-clpP_p5	ACTTCTTAAACGGCGAGGTC	ACGCCCAAGTTCATGGAATT	50.0	45.0	57.9	58.1	948 bp	0.5	58.8