Hotspot

ndhJ-ndhK

LSC · 56460-56611 · 152 bp

Back to Pithecellobium

Pi 0.027

MarkerSeek score 70.50

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 4
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	AGTTCAGTATCCTCCCACGA	50.0	50.0	58.2	57.5	209-213 bp	1.0	77.2
ndhJ-ndhK_p2	GCCAATCCTCGGGCTTTATT	AGTTCAGTATCCTCCCACGA	50.0	50.0	58.3	57.5	290-294 bp	1.0	75.5
ndhJ-ndhK_p3	GCCCATGTTTGACTAGCCAA	AGTTCAGTATCCTCCCACGA	50.0	50.0	58.5	57.5	221-225 bp	1.0	75.0
ndhJ-ndhK_p4	AATCGAATGCCAATCCTCGG	AGTTCAGTATCCTCCCACGA	50.0	50.0	58.4	57.5	298-302 bp	1.0	74.7
ndhJ-ndhK_p5	GCAATCGAATGCCAATCCTC	AGTTCAGTATCCTCCCACGA	50.0	50.0	57.6	57.5	300-304 bp	1.0	74.6