Hotspot

trnT-UGU-trnL-UAA

LSC · 52470-54085 · 1616 bp

Back to Pithecellobium

Pi 0.0312

MarkerSeek score 79.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1616
variable_sites: 45
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1532-1698 bp	1.0	84.3
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1540-1706 bp	1.0	83.7
trnT-UGU-trnL-UAA_p3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.4	58.0	1504-1670 bp	1.0	83.4
trnT-UGU-trnL-UAA_p4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.4	58.1	1512-1678 bp	1.0	82.8
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1546-1712 bp	1.0	82.4