Hotspot

psbC-trnS-UGA

LSC · 40894-41135 · 242 bp

Back to Pithecellobium

Pi 0.0496

MarkerSeek score 69.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 12
pi: 0.0496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CAGCGGGATTTGAAAAAGGC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	333 bp	1.0	78.9
psbC-trnS-UGA_p2	GCAGCGGGATTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	334 bp	1.0	78.8
psbC-trnS-UGA_p3	GCGGGATTTGAAAAAGGCAT	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	331 bp	1.0	78.1
psbC-trnS-UGA_p4	TGCAGCGGGATTTGAAAAAG	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	335 bp	1.0	77.6
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.4	58.2	372 bp	1.0	77.5